Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins.
نویسندگان
چکیده
Bacillus subtilis contains urease structural genes but lacks the accessory genes typically required for GTP-dependent incorporation of nickel. Nevertheless, B. subtilis was shown to possess a functional urease, and the recombinant enzyme conferred low levels of nickel-dependent activity to Escherichia coli. Additional investigations of the system lead to the suggestion that B. subtilis may use unidentified accessory proteins for in vivo urease activation.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 187 20 شماره
صفحات -
تاریخ انتشار 2005